Garrett A. Perchetti, Research Scientist - Virology
Research Scientist and MD Candidate in virology with publications on infectious diseases, laboratory medicine, viral genetics, and clinical medicine
Research Expertise
About
Publications
Comparative Performance of SARS-CoV-2 Detection Assays using Seven Different Primer/Probe Sets and One Assay Kit
Mar 17, 2020
Casto, A. M., Huang, M.-L., Nalla, A., Perchetti, G., Sampoleo, R., Shrestha, L., Wei, Y., Zhu, H., Greninger, A. L., & Jerome, K. R. (2020). Comparative Performance of SARS-CoV-2 Detection Assays using Seven Different Primer/Probe Sets and One Assay Kit. https://doi.org/10.1101/2020.03.13.20035618
Direct RT-qPCR detection of SARS-CoV-2 RNA from patient nasopharyngeal swabs without an RNA extraction step
PLOS Biology / Oct 02, 2020
Bruce, E. A., Huang, M.-L., Perchetti, G. A., Tighe, S., Laaguiby, P., Hoffman, J. J., Gerrard, D. L., Nalla, A. K., Wei, Y., Greninger, A. L., Diehl, S. A., Shirley, D. J., Leonard, D. G. B., Huston, C. D., Kirkpatrick, B. D., Dragon, J. A., Crothers, J. W., Jerome, K. R., & Botten, J. W. (2020). Direct RT-qPCR detection of SARS-CoV-2 RNA from patient nasopharyngeal swabs without an RNA extraction step. PLOS Biology, 18(10), e3000896. https://doi.org/10.1371/journal.pbio.3000896
Analytical Sensitivity of the Abbott BinaxNOW COVID-19 Ag Card
Journal of Clinical Microbiology / Feb 18, 2021
Perchetti, G. A., Huang, M.-L., Mills, M. G., Jerome, K. R., & Greninger, A. L. (2021). Analytical Sensitivity of the Abbott BinaxNOW COVID-19 Ag Card. Journal of Clinical Microbiology, 59(3). https://doi.org/10.1128/jcm.02880-20
Validation of SARS-CoV-2 detection across multiple specimen types
Journal of Clinical Virology / Jul 01, 2020
Perchetti, G. A., Nalla, A. K., Huang, M.-L., Zhu, H., Wei, Y., Stensland, L., Loprieno, M. A., Jerome, K. R., & Greninger, A. L. (2020). Validation of SARS-CoV-2 detection across multiple specimen types. Journal of Clinical Virology, 128, 104438. https://doi.org/10.1016/j.jcv.2020.104438
Predicting infectivity: comparing four PCR‐based assays to detect culturable SARS‐CoV‐2 in clinical samples
EMBO Molecular Medicine / Dec 13, 2021
Bruce, E. A., Mills, M. G., Sampoleo, R., Perchetti, G. A., Huang, M., Despres, H. W., Schmidt, M. M., Roychoudhury, P., Shirley, D. J., Jerome, K. R., Greninger, A. L., & Botten, J. W. (2021). Predicting infectivity: comparing four PCR‐based assays to detect culturable SARS‐CoV‐2 in clinical samples. EMBO Molecular Medicine, 14(2). Portico. https://doi.org/10.15252/emmm.202115290
20. Risk Factors for Breakthrough Cytomegalovirus (CMV) Infection and De Novo Resistance in Hematopoietic Cell Transplantation (HCT) Recipients Receiving Letermovir Prophylaxis
Open Forum Infectious Diseases / Nov 01, 2021
Zamora, D., Perchetti, G., Biernacki, M., Xie, H., Castor, J. L., Joncas-schronce, L., Blazevic, R., Leisenring, W., Huang, M.-L., Jerome, K., Martin, P. J., Boeckh, M., & Greninger, A. L. (2021). 20. Risk Factors for Breakthrough Cytomegalovirus (CMV) Infection and De Novo Resistance in Hematopoietic Cell Transplantation (HCT) Recipients Receiving Letermovir Prophylaxis. Open Forum Infectious Diseases, 8(Supplement_1), S13–S14. https://doi.org/10.1093/ofid/ofab466.020
Positive selection underlies repeated knockout of ORF8 in SARS-CoV-2 evolution
Nature Communications / Apr 13, 2024
Wagner, C., Kistler, K. E., Perchetti, G. A., Baker, N., Frisbie, L. A., Torres, L. M., Aragona, F., Yun, C., Figgins, M., Greninger, A. L., Cox, A., Oltean, H. N., Roychoudhury, P., & Bedford, T. (2024). Positive selection underlies repeated knockout of ORF8 in SARS-CoV-2 evolution. Nature Communications, 15(1). https://doi.org/10.1038/s41467-024-47599-5
Positive selection underlies repeated knockout of ORF8 in SARS-CoV-2 evolution
Sep 23, 2023
Wagner, C., Kistler, K. E., Perchetti, G. A., Baker, N., Frisbie, L. A., Torres, L. M., Aragona, F., Yun, C., Figgins, M., Greninger, A. L., Cox, A., Oltean, H. N., Roychoudhury, P., & Bedford, T. (2023). Positive selection underlies repeated knockout of ORF8 in SARS-CoV-2 evolution. https://doi.org/10.1101/2023.09.21.23295927
Cytomegalovirus breakthrough and resistance during letermovir prophylaxis
Bone Marrow Transplantation / Jan 24, 2023
Perchetti, G. A., Biernacki, M. A., Xie, H., Castor, J., Joncas-Schronce, L., Ueda Oshima, M., Kim, Y., Jerome, K. R., Sandmaier, B. M., Martin, P. J., Boeckh, M., Greninger, A. L., & Zamora, D. (2023). Cytomegalovirus breakthrough and resistance during letermovir prophylaxis. Bone Marrow Transplantation, 58(4), 430–436. https://doi.org/10.1038/s41409-023-01920-w
Rapid and accurate identification of SARS-CoV-2 Omicron variants using droplet digital PCR (RT-ddPCR)
Journal of Clinical Virology / Sep 01, 2022
Mills, M. G., Hajian, P., Bakhash, S. M., Xie, H., Mantzke, D., Zhu, H., Perchetti, G. A., Huang, M.-L., Pepper, G., Jerome, K. R., Roychoudhury, P., & Greninger, A. L. (2022). Rapid and accurate identification of SARS-CoV-2 Omicron variants using droplet digital PCR (RT-ddPCR). Journal of Clinical Virology, 154, 105218. https://doi.org/10.1016/j.jcv.2022.105218
Specific allelic discrimination of N501Y and other SARS‐CoV‐2 mutations by ddPCR detects B.1.1.7 lineage in Washington State
Journal of Medical Virology / Jul 03, 2021
Perchetti, G. A., Zhu, H., Mills, M. G., Shrestha, L., Wagner, C., Bakhash, S. M., Lin, M. J., Xie, H., Huang, M., Mathias, P., Bedford, T., Jerome, K. R., Greninger, A. L., & Roychoudhury, P. (2021). Specific allelic discrimination of N501Y and other SARS‐CoV‐2 mutations by ddPCR detects B.1.1.7 lineage in Washington State. Journal of Medical Virology, 93(10), 5931–5941. Portico. https://doi.org/10.1002/jmv.27155
A SARS-CoV-2 Nucleocapsid Variant that Affects Antigen Test Performance
Journal of Clinical Virology / Aug 01, 2021
Bourassa, L., Perchetti, G. A., Phung, Q., Lin, M. J., Mills, M. G., Roychoudhury, P., Harmon, K. G., Reed, J. C., & Greninger, A. L. (2021). A SARS-CoV-2 Nucleocapsid Variant that Affects Antigen Test Performance. Journal of Clinical Virology, 141, 104900. https://doi.org/10.1016/j.jcv.2021.104900
Predicting Infectivity: Comparing Four PCR-based Assays to Detect Culturable SARS-CoV-2 in Clinical Samples
Jul 18, 2021
Bruce, E. A., Mills, M. G., Sampoleo, R., Perchetti, G. A., Huang, M.-L., Despres, H. W., Shirley, D. J., Jerome, K. R., Greninger, A. L., & Botten, J. W. (2021). Predicting Infectivity: Comparing Four PCR-based Assays to Detect Culturable SARS-CoV-2 in Clinical Samples. https://doi.org/10.1101/2021.07.14.21260544
Performance characteristics of the Abbott Alinity m SARS-CoV-2 assay
Journal of Clinical Virology / Jul 01, 2021
Perchetti, G. A., Pepper, G., Shrestha, L., LaTurner, K., Yae Kim, D., Huang, M.-L., Jerome, K. R., & Greninger, A. L. (2021). Performance characteristics of the Abbott Alinity m SARS-CoV-2 assay. Journal of Clinical Virology, 140, 104869. https://doi.org/10.1016/j.jcv.2021.104869
A SARS-CoV-2 Nucleocapsid Variant that Affects Antigen Test Performance
May 05, 2021
Bourassa, L., Perchetti, G. A., Phung, Q., Lin, M. J., Mills, M. G., Roychoudhury, P., Harmon, K. G., Reed, J. C., & Greninger, A. L. (2021). A SARS-CoV-2 Nucleocapsid Variant that Affects Antigen Test Performance. https://doi.org/10.1101/2021.05.05.21256527
Human Metapneumovirus Infection and Genotyping of Infants in Rural Nepal
Journal of the Pediatric Infectious Diseases Society / Nov 02, 2020
Perchetti, G. A., Wilcox, N., Chu, H. Y., Katz, J., Khatry, S. K., LeClerq, S. C., Tielsch, J. M., Jerome, K. R., Englund, J. A., & Kuypers, J. (2020). Human Metapneumovirus Infection and Genotyping of Infants in Rural Nepal. Journal of the Pediatric Infectious Diseases Society, 10(4), 408–416. https://doi.org/10.1093/jpids/piaa118
Pooling of SARS-CoV-2 samples to increase molecular testing throughput
Journal of Clinical Virology / Oct 01, 2020
Perchetti, G. A., Sullivan, K.-W., Pepper, G., Huang, M.-L., Breit, N., Mathias, P., Jerome, K. R., & Greninger, A. L. (2020). Pooling of SARS-CoV-2 samples to increase molecular testing throughput. Journal of Clinical Virology, 131, 104570. https://doi.org/10.1016/j.jcv.2020.104570
Multiplexing primer/probe sets for detection of SARS-CoV-2 by qRT-PCR
Journal of Clinical Virology / Aug 01, 2020
Perchetti, G. A., Nalla, A. K., Huang, M.-L., Jerome, K. R., & Greninger, A. L. (2020). Multiplexing primer/probe sets for detection of SARS-CoV-2 by qRT-PCR. Journal of Clinical Virology, 129, 104499. https://doi.org/10.1016/j.jcv.2020.104499
Detection of SARS-CoV-2 by bronchoscopy after negative nasopharyngeal testing: Stay vigilant for COVID-19
Respiratory Medicine Case Reports / Jan 01, 2020
Ramos, K. J., Kapnadak, S. G., Collins, B. F., Pottinger, P. S., Wall, R., Mays, J. A., Perchetti, G. A., Jerome, K. R., Khot, S., Limaye, A. P., Mathias, P. C., & Greninger, A. (2020). Detection of SARS-CoV-2 by bronchoscopy after negative nasopharyngeal testing: Stay vigilant for COVID-19. Respiratory Medicine Case Reports, 30, 101120. https://doi.org/10.1016/j.rmcr.2020.101120
Stability of SARS-CoV-2 in Phosphate-Buffered Saline for Molecular Detection
Journal of Clinical Microbiology / Jul 23, 2020
Perchetti, G. A., Huang, M.-L., Peddu, V., Jerome, K. R., & Greninger, A. L. (2020). Stability of SARS-CoV-2 in Phosphate-Buffered Saline for Molecular Detection. Journal of Clinical Microbiology, 58(8). https://doi.org/10.1128/jcm.01094-20
Metagenomic Analysis Reveals Clinical SARS-CoV-2 Infection and Bacterial or Viral Superinfection and Colonization
Clinical Chemistry / Jun 12, 2020
Peddu, V., Shean, R. C., Xie, H., Shrestha, L., Perchetti, G. A., Minot, S. S., Roychoudhury, P., Huang, M.-L., Nalla, A., Reddy, S. B., Phung, Q., Reinhardt, A., Jerome, K. R., & Greninger, A. L. (2020). Metagenomic Analysis Reveals Clinical SARS-CoV-2 Infection and Bacterial or Viral Superinfection and Colonization. Clinical Chemistry, 66(7), 966–972. https://doi.org/10.1093/clinchem/hvaa106
Comparative Performance of SARS-CoV-2 Detection Assays Using Seven Different Primer-Probe Sets and One Assay Kit
Journal of Clinical Microbiology / May 26, 2020
Nalla, A. K., Casto, A. M., Huang, M.-L. W., Perchetti, G. A., Sampoleo, R., Shrestha, L., Wei, Y., Zhu, H., Jerome, K. R., & Greninger, A. L. (2020). Comparative Performance of SARS-CoV-2 Detection Assays Using Seven Different Primer-Probe Sets and One Assay Kit. Journal of Clinical Microbiology, 58(6). https://doi.org/10.1128/jcm.00557-20
DIRECT RT-qPCR DETECTION OF SARS-CoV-2 RNA FROM PATIENT NASOPHARYNGEAL SWABS WITHOUT AN RNA EXTRACTION STEP
Mar 21, 2020
Bruce, E. A., Huang, M.-L., Perchetti, G. A., Tighe, S., Laaguiby, P., Hoffman, J. J., Gerrard, D. L., Nalla, A. K., Wei, Y., Greninger, A. L., Diehl, S. A., Shirley, D. J., Leonard, D. G. B., Huston, C. D., Kirkpatrick, B. D., Dragon, J. A., Crothers, J. W., Jerome, K. R., & Botten, J. W. (2020). DIRECT RT-qPCR DETECTION OF SARS-CoV-2 RNA FROM PATIENT NASOPHARYNGEAL SWABS WITHOUT AN RNA EXTRACTION STEP. https://doi.org/10.1101/2020.03.20.001008
Phylogenetic characterization of rhinoviruses from infants in Sarlahi, Nepal
Journal of Medical Virology / Aug 21, 2019
Kuypers, J., Perchetti, G. A., Chu, H. Y., Newman, K. L., Katz, J., Khatry, S. K., LeClerq, S. C., Jerome, K. R., Tielsch, J. M., & Englund, J. A. (2019). Phylogenetic characterization of rhinoviruses from infants in Sarlahi, Nepal. Journal of Medical Virology, 91(12), 2108–2116. Portico. https://doi.org/10.1002/jmv.25563
2626. Rhinovirus in Children Presenting to the Emergency Department: Role of Viral Load in Disease Severity and Co-Infections
Open Forum Infectious Diseases / Oct 01, 2019
Waghmare, A., Strelitz, B., Lacombe, K., Perchetti, G., Nalla, A., Rha, B., Midgley, C., Lively, J. Y., Klein, E. J., Kuypers, J., & Englund, J. A. (2019). 2626. Rhinovirus in Children Presenting to the Emergency Department: Role of Viral Load in Disease Severity and Co-Infections. Open Forum Infectious Diseases, 6(Supplement_2), S915–S916. https://doi.org/10.1093/ofid/ofz360.2304
Large, Stable, Contemporary Interspecies Recombination Events in Circulating Human Herpes Simplex Viruses
The Journal of Infectious Diseases / Apr 23, 2019
Casto, A. M., Roychoudhury, P., Xie, H., Selke, S., Perchetti, G. A., Wofford, H., Huang, M.-L., Verjans, G. M. G. M., Gottlieb, G. S., Wald, A., Jerome, K. R., Koelle, D. M., Johnston, C., & Greninger, A. L. (2019). Large, Stable, Contemporary Interspecies Recombination Events in Circulating Human Herpes Simplex Viruses. The Journal of Infectious Diseases. https://doi.org/10.1093/infdis/jiz199
Specific allelic discrimination of N501Y and other SARS-CoV-2 mutations by ddPCR detects B.1.1.7 lineage in Washington State
Mar 12, 2021
Perchetti, G. A., Zhu, H., Mills, M. G., Shrestha, L., Wagner, C., Bakhash, S. M., Lin, M., Xie, H., Huang, M.-L., Mathias, P., Bedford, T., Jerome, K. R., Greninger, A. L., & Roychoudhury, P. (2021). Specific allelic discrimination of N501Y and other SARS-CoV-2 mutations by ddPCR detects B.1.1.7 lineage in Washington State. https://doi.org/10.1101/2021.03.10.21253321
The impact of interspecies recombination on human herpes simplex virus evolution and host immune recognition
Nov 19, 2018
Casto, A. M., Roychoudhury, P., Xie, H., Selke, S., Perchetti, G. A., Wofford, H., Huang, M.-L., Verjans, G. M. G. M., Gottlieb, G. S., Wald, A., Jerome, K. R., Koelle, D. M., Johnston, C., & Greninger, A. L. (2018). The impact of interspecies recombination on human herpes simplex virus evolution and host immune recognition. https://doi.org/10.1101/472639
Education
University of Washington
BS, BA, Microbiology; Art History
Ross University School of Medicine
MD, Infectious Diseases / April, 2025 (anticipated)
Harvard University
SSP, Psychology
Experience
University of Washington
Research Scientist / 2015 — Present
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