Murli Manohar
Boyce Thompson Institute, Cornell University
Research Expertise
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Education
Texas A&M University
Biochemistry, Plant Biology, Biotechnology, Molecular Biology / May, 2012
Indian Institute of Technology Roorkee
M.S, Biotechnology / May, 2005
B.S, Agriculture / May, 2003
Experience
Ascribe Bioscience Inc.
CTO / February, 2019 — Present
Execute the company's technological roadmap in alignment with business goals and vision. Collaborate with cross-functional teams to drive innovation and enhance crop protection products. Analyze market trends and competitive landscape to identify new technological advancements and product differentiation opportunities. ● Built strong partnerships with Ag-tech firms, investors, academic scholars, and growers. ● Controlled research and development budget of more than $10M to drive innovation into tangible outcomes. ● Developed independent research laboratory and managed R&D, multi-national field tests, and commercialization plans. ● Conducted market research to enhance product offerings and align with customer requirements.
Boyce Thompson Institute for Plant Research
Senior Scientist / September, 2012 — November, 2020
Supported multidisciplinary teams in assessing complex data sets, identifying patterns, and drawing meaningful conclusions. Led design and execution of experiments, ensuring adherence to scientific protocols and research methodologies. Facilitated collaborative research projects and leveraged resources for mutual benefit. ● Recognized as the inventor of several technologies, delivering a foundation for establishing Ascribe Bioscience. ● Uncovered unique processes of plant and animal responses to external stresses. ● Earned promotion to Senior Scientist in 2017 based on excellent performance. ● Expert in the fields of Molecular Biology, Plant Biotechnology, Crop Protection, and Gene Editing. ● Authored revolutionary research publications in Agriculture, Molecular Biology, Biochemistry, Biotechnology, and Natural Chemistry.
Baylor College of Medicine
Graduate Research Assistant/Postdoctoral Associate / May, 2008 — September, 2012
The objective was to study and optimize CAX-mediated biofortification and phytoremediation in plants. My research investigated the physiological functions, structure-function relationship, and inter- and intra-molecular interactions of calcium transporters cation/proton exchangers known as CAX in plants. My research used Saccharomyces cerevisiae (yeast) as the model system to study CAX transporters and established membrane fractionation and enrichment techniques to evaluate the real-time movement of metals between membranes. Moreover, I also characterized new calcium transporters from both plants and animals. Other experience: Hands-on experience in executing potency assays (binding assays, In vitro, In vivo). Hands-on experience in RNAi technology (Designing, cloning, and heterologous expression of siRNA in plants targeting animal mRNA).
The University of Texas Health Science Center at Houston School of Public Health
Visiting Research Student / September, 2010 — September, 2012
My work objective was to express and purify CAX (Calcium transporter) proteins through heterologous expression and determine their three-dimensional structure using X-ray crystallography. The work involved cloning 15+ CAX proteins from different plant species in E. coli and yeast expression vectors. It also included optimizing and expressing protein production, screening and solubilizing proteins using detergents, and purifying functional proteins through affinity chromatography. The metal transport activity of purified proteins was evaluated by reconstituting the proteins in proteoliposomes, isothermal titration calorimetry (ITC), and in inverted vesicles prepared from protein-expressing E. coli cells. The purified protein underwent crystallization screening to assess the effectiveness of methods, reagents, and other variables in producing high-quality crystals of CAX proteins for 3D structure analysis.
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