Ranjithmenon Muraleedharan

Broad Institute of MIT and Harvard

Research Expertise

Cancer Biology
Cell Biology
Immunology
Immunology and Allergy
Molecular Biology
Biochemistry
Genetics
Genetics (clinical)
Cancer Research
Neurology (clinical)
Oncology
Neurology

About

I am a Scientist specializing in phenotypic assay development and screening. We have several high content screening platforms and a range of liquid handlers, all automated for 96 & 384-well plates. We carry out feasibility studies, assay development and automated Drug screening. I am consulting hundreds of researchers from Broad Institute and other Harvard and MIT affiliates, training and helping them with imaging and image analysis. xperience & Capabilities cover: <br> \- High content imaging CRISPR screens \(gene silencing & gene editing\)\. \- High Content Imaging as an Early Drug Discovery Tool \(Small molecule Library screens\)\. \- Live cultures\, including live co\-cultures\. \- Neuronal assays\, including neuronal co\-cultures\, chemotaxis\, and neurotoxicity assays\. \- iPSC \-derived assays including differentiation and regeneration\. \- 3D cultures\, like Neurospheres\. \- Any multiplexed mechanism of action studies\, including co\-localization\, internalization\, morphology changes\. Software Capabilities: Harmony High-Content Imaging and Analysis Software, CellProfiler and CellProfiler Analyst, Spotfire, StratoMineR. Data management: AWS Cloud platform. Our systems produce over 3TB data/week. I manage our cloud services and investigate opportunities to reduce spend across the multi-cloud environments. #High Throughput Screening, #High-content imaging-based screens, #High throughput cell-based assay development, #Drug Development

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Education

University of Nottingham

Ph.D, Stem cell biology, Cancer Biology and Imaging / December, 2014

Nottingham

University of Nottingham

MSC, MOLECULAR MEDICAL MICROBIOLOGY / September, 2007

Nottingham

Experience

Cincinnati Children's Hospital Medical Center

Post-Doctoral Associate / May, 2014April, 2018

Yale University

Associate Research Scientist / May, 2018October, 2021

Broad Institute of MIT & Harvard

Research Scientist / October, 2021Present

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