Ajay Badhan
Research Biologist, Lethbridge Research Center, Canada
Research Expertise
About
Publications
Production of multiple xylanolytic and cellulolytic enzymes by thermophilic fungus Myceliophthora sp. IMI 387099
Bioresource Technology / Feb 01, 2007
Badhan, A. K., Chadha, B. S., Kaur, J., Saini, H. S., & Bhat, M. K. (2007). Production of multiple xylanolytic and cellulolytic enzymes by thermophilic fungus Myceliophthora sp. IMI 387099. Bioresource Technology, 98(3), 504–510. https://doi.org/10.1016/j.biortech.2006.02.009
Purification and characterization of ß-glucosidase from Melanocarpus sp. MTCC 3922
Electronic Journal of Biotechnology / Apr 15, 2007
Kaur, J., Chadha, B. S., Kumar, B. A., Ghatora, S. K., & Saini, H. S. (2007). Purification and characterization of ß-glucosidase from Melanocarpus sp. MTCC 3922. Electronic Journal of Biotechnology, 10(2), 0–0. https://doi.org/10.2225/vol10-issue2-fulltext-4
Development of Cellulosic Secondary Walls in Flax Fibers Requires β-Galactosidase
Plant Physiology / May 19, 2011
Roach, M. J., Mokshina, N. Y., Badhan, A., Snegireva, A. V., Hobson, N., Deyholos, M. K., & Gorshkova, T. A. (2011). Development of Cellulosic Secondary Walls in Flax Fibers Requires β-Galactosidase . Plant Physiology, 156(3), 1351–1363. https://doi.org/10.1104/pp.111.172676
Purification and characterization of two endoglucanases from Melanocarpus sp. MTCC 3922
Bioresource Technology / Jan 01, 2007
KAUR, J., CHADHA, B., KUMAR, B., & SAINI, H. (2007). Purification and characterization of two endoglucanases from Melanocarpus sp. MTCC 3922. Bioresource Technology, 98(1), 74–81. https://doi.org/10.1016/j.biortech.2005.11.019
Mining the rumen for fibrolytic feed enzymes
Animal Frontiers / Apr 01, 2016
Ribeiro, G. O., Gruninger, R. J., Badhan, A., & McAllister, T. A. (2016). Mining the rumen for fibrolytic feed enzymes. Animal Frontiers, 6(2), 20–26. https://doi.org/10.2527/af.2016-0019
Identification and characterization of diverse xylanases from thermophilic and thermotolerant fungi
BioResources / Jul 01, 2006
Ghatora, S. K., Chadha, B. S., Badhan, A. K., Saini, H. S., & Bhat, M. K. (2006). Identification and characterization of diverse xylanases from thermophilic and thermotolerant fungi. BioResources, 1(1), 18–33. https://doi.org/10.15376/biores.1.1.18-33
Functionally diverse multiple xylanases of thermophilic fungus Myceliophthora sp. IMI 387099
Enzyme and Microbial Technology / Oct 01, 2004
Badhan, A. K., Chadha, B. S., Sonia, K. G., Saini, H. S., & Bhat, M. K. (2004). Functionally diverse multiple xylanases of thermophilic fungus Myceliophthora sp. IMI 387099. Enzyme and Microbial Technology, 35(5), 460–466. https://doi.org/10.1016/j.enzmictec.2004.07.002
Expression of a fungal ferulic acid esterase in alfalfa modifies cell wall digestibility
Biotechnology for Biofuels / Mar 20, 2014
Badhan, A., Jin, L., Wang, Y., Han, S., Kowalczys, K., Brown, D. C., Ayala, C. J., Latoszek-Green, M., Miki, B., Tsang, A., & McAllister, T. (2014). Expression of a fungal ferulic acid esterase in alfalfa modifies cell wall digestibility. Biotechnology for Biofuels, 7(1). https://doi.org/10.1186/1754-6834-7-39
Fibre digestion by rumen microbiota — a review of recent metagenomic and metatranscriptomic studies
Canadian Journal of Animal Science / Dec 01, 2019
Terry, S. A., Badhan, A., Wang, Y., Chaves, A. V., & McAllister, T. A. (2019). Fibre digestion by rumen microbiota — a review of recent metagenomic and metatranscriptomic studies. Canadian Journal of Animal Science, 99(4), 678–692. https://doi.org/10.1139/cjas-2019-0024
Formulation of enzyme blends to maximize the hydrolysis of alkaline peroxide pretreated alfalfa hay and barley straw by rumen enzymes and commercial cellulases
BMC Biotechnology / Apr 26, 2014
Badhan, A., Wang, Y., Gruninger, R., Patton, D., Powlowski, J., Tsang, A., & McAllister, T. (2014). Formulation of enzyme blends to maximize the hydrolysis of alkaline peroxide pretreated alfalfa hay and barley straw by rumen enzymes and commercial cellulases. BMC Biotechnology, 14(1). https://doi.org/10.1186/1472-6750-14-31
Identification of glucose tolerant acid active β-glucosidases from thermophilic and thermotolerant fungi
World Journal of Microbiology and Biotechnology / Aug 23, 2007
Sonia, K. G., Chadha, B. S., Badhan, A. K., Saini, H. S., & Bhat, M. K. (2007). Identification of glucose tolerant acid active β-glucosidases from thermophilic and thermotolerant fungi. World Journal of Microbiology and Biotechnology, 24(5), 599–604. https://doi.org/10.1007/s11274-007-9512-6
Two endoxylanases active and stable at alkaline pH from the newly isolated thermophilic fungus, Myceliophthora sp. IMI 387099
Journal of Biotechnology / Apr 01, 2004
Chadha, B. S., Ajay, B. K., Mellon, F., & Bhat, M. K. (2004). Two endoxylanases active and stable at alkaline pH from the newly isolated thermophilic fungus, Myceliophthora sp. IMI 387099. Journal of Biotechnology, 109(3), 227–237. https://doi.org/10.1016/j.jbiotec.2003.12.010
New recombinant fibrolytic enzymes for improved in vitro ruminal fiber degradability of barley straw1
Journal of Animal Science / Jul 20, 2018
Ribeiro, G. O., Badhan, A., Huang, J., Beauchemin, K. A., Yang, W., Wang, Y., Tsang, A., & McAllister, T. A. (2018). New recombinant fibrolytic enzymes for improved in vitro ruminal fiber degradability of barley straw1. Journal of Animal Science, 96(9), 3928–3942. https://doi.org/10.1093/jas/sky251
Identification of novel enzymes to enhance the ruminal digestion of barley straw
Bioresource Technology / Jul 01, 2018
Badhan, A., Ribeiro, G. O., Jones, D. R., Wang, Y., Abbott, D. W., Di Falco, M., Tsang, A., & McAllister, T. A. (2018). Identification of novel enzymes to enhance the ruminal digestion of barley straw. Bioresource Technology, 260, 76–84. https://doi.org/10.1016/j.biortech.2018.03.086
Role of Transglycosylation Products in the Expression of Multiple Xylanases in Myceliophthora sp. IMI 387099
Current Microbiology / May 14, 2007
Badhan, A. K., Chadha, B. S., Kaur, J., Sonia, K. G., Saini, H. S., & Bhat, M. K. (2007). Role of Transglycosylation Products in the Expression of Multiple Xylanases in Myceliophthora sp. IMI 387099. Current Microbiology, 54(6), 405–409. https://doi.org/10.1007/s00284-006-0204-5
Designer Plants for Biofuels: A Review
Current Metabolomics / Mar 02, 2016
Badhan, A., & McAllister, T. (2016). Designer Plants for Biofuels: A Review. Current Metabolomics, 4(1), 49–55. https://doi.org/10.2174/2213235x03666141226213656
Purification of the alkaliphilic xylanases from Myceliophthora sp. IMI 387099 using cellulose-binding domain as an affinity tag
World Journal of Microbiology and Biotechnology / Sep 19, 2007
Badhan, A. K., Chadha, B. S., & Saini, H. S. (2007). Purification of the alkaliphilic xylanases from Myceliophthora sp. IMI 387099 using cellulose-binding domain as an affinity tag. World Journal of Microbiology and Biotechnology, 24(7), 973–981. https://doi.org/10.1007/s11274-007-9561-x
Mechanistic insights into the digestion of complex dietary fibre by the rumen microbiota using combinatorial high-resolution glycomics and transcriptomic analyses
Computational and Structural Biotechnology Journal / Jan 01, 2022
Badhan, A., Low, K. E., Jones, D. R., Xing, X., Milani, M. R. M., Polo, R. O., Klassen, L., Venketachalam, S., Hahn, M. G., Abbott, D. W., & McAllister, T. A. (2022). Mechanistic insights into the digestion of complex dietary fibre by the rumen microbiota using combinatorial high-resolution glycomics and transcriptomic analyses. Computational and Structural Biotechnology Journal, 20, 148–164. https://doi.org/10.1016/j.csbj.2021.12.009
Improvement in Saccharification Yield of Mixed Rumen Enzymes by Identification of Recalcitrant Cell Wall Constituents Using Enzyme Fingerprinting
BioMed Research International / Jan 01, 2015
Badhan, A., Wang, Y.-X., Gruninger, R., Patton, D., Powlowski, J., Tsang, A., & McAllister, T. A. (2015). Improvement in Saccharification Yield of Mixed Rumen Enzymes by Identification of Recalcitrant Cell Wall Constituents Using Enzyme Fingerprinting. BioMed Research International, 2015, 1–13. https://doi.org/10.1155/2015/562952
Proteomic Profiling of the Aleurone Layer of Mature Arabidopsis thaliana Seed
Plant Molecular Biology Reporter / Aug 07, 2012
Rashid, A., Badhan, A., Deyholos, M., & Kav, N. (2012). Proteomic Profiling of the Aleurone Layer of Mature Arabidopsis thaliana Seed. Plant Molecular Biology Reporter, 31(2), 464–469. https://doi.org/10.1007/s11105-012-0498-3
Saccharification efficiencies of multi-enzyme complexes produced by aerobic fungi
New Biotechnology / Nov 01, 2018
Badhan, A., Huang, J., Wang, Y., Abbott, D. W., Di Falco, M., Tsang, A., & McAllister, T. (2018). Saccharification efficiencies of multi-enzyme complexes produced by aerobic fungi. New Biotechnology, 46, 1–6. https://doi.org/10.1016/j.nbt.2018.05.003
Analysis of Complex Carbohydrate Composition in Plant Cell Wall Using Fourier Transformed Mid-Infrared Spectroscopy (FT-IR)
Methods in Molecular Biology / Jan 01, 2017
Badhan, A., Wang, Y., & McAllister, T. A. (2017). Analysis of Complex Carbohydrate Composition in Plant Cell Wall Using Fourier Transformed Mid-Infrared Spectroscopy (FT-IR). In Protein-Carbohydrate Interactions (pp. 209–214). Springer New York. https://doi.org/10.1007/978-1-4939-6899-2_16
Current Knowledge and Future Opportunities for Ruminant Enzymes
Enzymes in Farm Animal Nutrition / Mar 11, 2022
Rosser, C., Terry, S. A., Badhan, A., McAllister, T. A., & Beauchemin, K. A. (2022). Current Knowledge and Future Opportunities for Ruminant Enzymes. In Enzymes in Farm Animal Nutrition (pp. 153–169). CABI. https://doi.org/10.1079/9781789241563.0009
Blue Native Page in Combination with Mass Spectrometry as an Effective Tool to Study Multi-Enzyme Complex’s in Secretome of Aerobic Fungus
Annals of Chromatography and Separation Techniques / Jan 01, 2016
Badhan, A. (2016). Blue Native Page in Combination with Mass Spectrometry as an Effective Tool to Study Multi-Enzyme Complex’s in Secretome of Aerobic Fungus. Annals of Chromatography and Separation Techniques, 2(2), 1–2. https://doi.org/10.36876/acst.1019
From Plant Cell Walls to Biofuels—Arabidopsis thaliana Model
Handbook of Bioenergy Crop Plants / Mar 22, 2012
Pattathil, S., Avci, U., Biswal, A., & Badhan, A. (2012). From Plant Cell Walls to Biofuels—Arabidopsis thaliana Model. In Handbook of Bioenergy Crop Plants (pp. 75–97). CRC Press. https://doi.org/10.1201/b11711-7
Comparative analysis of functional diversity of rumen microbiome in bison and beef heifers
Applied and Environmental Microbiology / Dec 21, 2023
Nguyen, T. T. M., Badhan, A. K., Reid, I. D., Ribeiro, G., Gruninger, R., Tsang, A., Guan, L. L., & McAllister, T. (2023). Comparative analysis of functional diversity of rumen microbiome in bison and beef heifers. Applied and Environmental Microbiology, 89(12). https://doi.org/10.1128/aem.01320-23
Analysis of Complex Carbohydrate Composition in Plant Cell Wall Using Fourier Transform Mid-Infrared Spectroscopy
Methods in Molecular Biology / Jan 01, 2023
Badhan, A., Wang, Y., & McAllister, T. A. (2023). Analysis of Complex Carbohydrate Composition in Plant Cell Wall Using Fourier Transform Mid-Infrared Spectroscopy. In Carbohydrate-Protein Interactions (pp. 207–213). Springer US. https://doi.org/10.1007/978-1-0716-3151-5_15
New mechanistic insight into the digestion of complex dietary fibre by rumen microbiota using combinatorial high-resolution glycomic and transcriptomic analyses
Nov 13, 2020
Badhan, A., Low, K., Jones, D., Xing, X., Milani, M. R. M., OrtegoPolo, R., Abbott, D. W., Venketachalam, S., Hahn, M., & McAllister, T. (2020). New mechanistic insight into the digestion of complex dietary fibre by rumen microbiota using combinatorial high-resolution glycomic and transcriptomic analyses. https://doi.org/10.21203/rs.3.rs-104578/v1
PSX-15 Screening recombinant fibrolytic enzymes for improved ruminal fiber degradability of barley straw.
Journal of Animal Science / Dec 01, 2018
Ribeiro, G., Badhan, A., Huang, J., Beauchemin, K., Yang, W., Wang, Y., Tsang, A., & McAllister, T. (2018). PSX-15 Screening recombinant fibrolytic enzymes for improved ruminal fiber degradability of barley straw. Journal of Animal Science, 96(suppl_3), 413–414. https://doi.org/10.1093/jas/sky404.906
Education
Ph.D., Microbiology
Experience
Agriculture Agri-food Canada
Biologist / May, 2015 — Present
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