Yonghong Zhao

Motivated and result-oriented biophysicist with 15+ years of experience in drug discovery and development

Research Expertise

Biologic drug
protein structure
bioinformatics
Biotechnology
Biochemistry
Molecular Biology
Biophysics
Cell Biology
Immunology
Structural Biology
Molecular Medicine
Pharmacology
Genetics

About

**Motivated and result-oriented biophysicist / structural biologist / protein scientist with 15+ years of experience in drug discovery and development**. Skills and Area of Expertise: **Biophysics** : SPR, ITC, SEC-MALS, HPLC, DLS, LS/MS, spectroscopy, radiation **Structural Biology**: protein structure-function relationship, X-ray crystallography **Protein Science**: recombinant protein expression, protein purification, bioconjugation techniques **Drug Modalities**: biologics (monoclonal antibody, bispecific antibody, and antibody drug conjugate), cell therapy, and molecular glue (target protein degradation) **Assay Development & Drug Discovery**: biochemistry fundamentals, assay design and optimization, photonics and imaging, enzyme kinetics, quality control and validation, hit generation, lead optimization Team Leadership & Collaboration: head of functional groups, project leader in matrix teams, department representative in compound development teams (CDT)

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Publications

Structure of Microsomal Cytochrome P450 2B4 Complexed with the Antifungal Drug Bifonazole
Journal of Biological Chemistry
2006
Two routes for production and purification of Fab fragments in biopharmaceutical discovery research: Papain digestion of mAb and transient expression in mammalian cells
Protein Expression and Purification
2009
Structure–function analysis of cytochromes P450 2B
Biochimica et Biophysica Acta (BBA) - General Subjects
2007
IgG2 Fc structure and the dynamic features of the IgG CH2–CH3 interface
Molecular Immunology
2013
Lateral Clustering of TLR3:dsRNA Signaling Units Revealed by TLR3ecd:3Fabs Quaternary Structure
Journal of Molecular Biology
2012
Structural and Thermodynamic Consequences of 1-(4-Chlorophenyl)imidazole Binding to Cytochrome P450 2B4,
Biochemistry
2007
Human Framework Adaptation of a Mouse Anti-Human IL-13 Antibody
Journal of Molecular Biology
2010
Rational Engineering of Human Cytochrome P450 2B6 for Enhanced Expression and Stability: Importance of a Leu264→Phe Substitution
Molecular Pharmacology
2007
The crystal structure of Escherichia coli heat shock protein YedU reveals three potential catalytic active sites
Protein Science
2003
The crystal structure of the E. coli stress protein YciF
Protein Science
2006
Structure of the EMMPRIN N‐terminal domain 1: Dimerization via β‐strand swapping
Proteins: Structure, Function, and Bioinformatics
2009
Coevolution of Antibody Stability and Vκ CDR-L3 Canonical Structure
Journal of Molecular Biology
2010
Crystal structure of tissue factor in complex with antibody 10H10 reveals the signaling epitope
Cellular Signalling
2017
On the domain pairing in chimeric antibodies
Molecular Immunology
2010
Escherichia colistress protein YciF: expression, crystallization and preliminary crystallographic analysis
Acta Crystallographica Section D Biological Crystallography
2004
His‐tag binding by antibody C706 mimics β‐amyloid recognition
Journal of Molecular Recognition
2010
Expression, crystallization and preliminary crystallographic analysis of YciE, a stress protein fromEscherichia coli
Acta Crystallographica Section D Biological Crystallography
2004
Conformational plasticity of cytochrome P450 2B4: crystallographic and solution studies
The FASEB Journal
2006
Crystal structure of Escherichia coli heat shock protein YedU
Unknown Venue
2004

Education

The University of Texas Medical Branch at Galveston

Ph.D., Biophysics / January, 2005

Galveston, Texas, United States of America

Experience

Transcenta Therapeutics

SEED Therapeutics

Incyte

Janssen Pharmaceuticals

Centocor

The University of Texas Medical Branch (UTMB)

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