Jordan Woehl, Ph.D.
Scripps Research/Calibr
Research Expertise
About
Publications
The Extracellular Adherence Protein from Staphylococcus aureus Inhibits the Classical and Lectin Pathways of Complement by Blocking Formation of the C3 Proconvertase
The Journal of Immunology / Dec 15, 2014
Woehl, J. L., Stapels, D. A. C., Garcia, B. L., Ramyar, K. X., Keightley, A., Ruyken, M., Syriga, M., Sfyroera, G., Weber, A. B., Zolkiewski, M., Ricklin, D., Lambris, J. D., Rooijakkers, S. H. M., & Geisbrecht, B. V. (2014). The Extracellular Adherence Protein from Staphylococcus aureus Inhibits the Classical and Lectin Pathways of Complement by Blocking Formation of the C3 Proconvertase. The Journal of Immunology, 193(12), 6161–6171. https://doi.org/10.4049/jimmunol.1401600
1H, 15N, and 13C resonance assignments of Staphylococcus aureus extracellular adherence protein domain 4
Biomolecular NMR Assignments / Jul 02, 2016
Woehl, J. L., Takahashi, D., Herrera, A. I., Geisbrecht, B. V., & Prakash, O. (2016). 1H, 15N, and 13C resonance assignments of Staphylococcus aureus extracellular adherence protein domain 4. Biomolecular NMR Assignments, 10(2), 301–305. https://doi.org/10.1007/s12104-016-9688-5
The structural basis for inhibition of the classical and lectin complement pathways by S. aureus extracellular adherence protein
Protein Science / May 31, 2017
Woehl, J. L., Ramyar, K. X., Katz, B. B., Walker, J. K., & Geisbrecht, B. V. (2017). The structural basis for inhibition of the classical and lectin complement pathways by S. aureus extracellular adherence protein. Protein Science, 26(8), 1595–1608. Portico. https://doi.org/10.1002/pro.3195
Identification of peptidic inhibitors of the alternative complement pathway based on Staphylococcus aureus SCIN proteins
Molecular Immunology / Oct 01, 2015
Summers, B. J., Garcia, B. L., Woehl, J. L., Ramyar, K. X., Yao, X., & Geisbrecht, B. V. (2015). Identification of peptidic inhibitors of the alternative complement pathway based on Staphylococcus aureus SCIN proteins. Molecular Immunology, 67(2), 193–205. https://doi.org/10.1016/j.molimm.2015.05.012
Evidence for multiple modes of neutrophil serine protease recognition by the EAP family of Staphylococcal innate immune evasion proteins
Protein Science / Nov 21, 2017
Stapels, D. A. C., Woehl, J. L., Milder, F. J., Tromp, A. T., van Batenburg, A. A., de Graaf, W. C., Broll, S. C., White, N. M., Rooijakkers, S. H. M., & Geisbrecht, B. V. (2017). Evidence for multiple modes of neutrophil serine protease recognition by the EAP family of Staphylococcal innate immune evasion proteins. Protein Science, 27(2), 509–522. Portico. https://doi.org/10.1002/pro.3342
An Irreversible Inhibitor to Probe the Role of Streptococcus pyogenes Cysteine Protease SpeB in Evasion of Host Complement Defenses
ACS Chemical Biology / Jul 14, 2020
Woehl, J. L., Kitamura, S., Dillon, N., Han, Z., Edgar, L. J., Nizet, V., & Wolan, D. W. (2020). An Irreversible Inhibitor to Probe the Role of Streptococcus pyogenes Cysteine Protease SpeB in Evasion of Host Complement Defenses. ACS Chemical Biology, 15(8), 2060–2069. https://doi.org/10.1021/acschembio.0c00191
An influenza A hemagglutinin small-molecule fusion inhibitor identified by a new high-throughput fluorescence polarization screen
Proceedings of the National Academy of Sciences / Jul 20, 2020
Yao, Y., Kadam, R. U., Lee, C.-C. D., Woehl, J. L., Wu, N. C., Zhu, X., Kitamura, S., Wilson, I. A., & Wolan, D. W. (2020). An influenza A hemagglutinin small-molecule fusion inhibitor identified by a new high-throughput fluorescence polarization screen. Proceedings of the National Academy of Sciences, 117(31), 18431–18438. https://doi.org/10.1073/pnas.2006893117
SuFEx-enabled, agnostic discovery of covalent inhibitors of human neutrophil elastase
Proceedings of the National Academy of Sciences / Sep 04, 2019
Zheng, Q., Woehl, J. L., Kitamura, S., Santos-Martins, D., Smedley, C. J., Li, G., Forli, S., Moses, J. E., Wolan, D. W., & Sharpless, K. B. (2019). SuFEx-enabled, agnostic discovery of covalent inhibitors of human neutrophil elastase. Proceedings of the National Academy of Sciences, 116(38), 18808–18814. https://doi.org/10.1073/pnas.1909972116
Diversity oriented clicking delivers β-substituted alkenyl sulfonyl fluorides as covalent human neutrophil elastase inhibitors
Proceedings of the National Academy of Sciences / Sep 07, 2022
Cheng, Y., Li, G., Smedley, C. J., Giel, M.-C., Kitamura, S., Woehl, J. L., Bianco, G., Forli, S., Homer, J. A., Cappiello, J. R., Wolan, D. W., Moses, J. E., & Sharpless, K. B. (2022). Diversity oriented clicking delivers β-substituted alkenyl sulfonyl fluorides as covalent human neutrophil elastase inhibitors. Proceedings of the National Academy of Sciences, 119(37). https://doi.org/10.1073/pnas.2208540119
Sialic Acid Ligands of CD28 Suppress Costimulation of T Cells
ACS Central Science / Aug 23, 2021
Edgar, L. J., Thompson, A. J., Vartabedian, V. F., Kikuchi, C., Woehl, J. L., Teijaro, J. R., & Paulson, J. C. (2021). Sialic Acid Ligands of CD28 Suppress Costimulation of T Cells. ACS Central Science, 7(9), 1508–1515. https://doi.org/10.1021/acscentsci.1c00525
Sulfur(VI) Fluoride Exchange (SuFEx)-Enabled High-Throughput Medicinal Chemistry
Journal of the American Chemical Society / Jun 01, 2020
Kitamura, S., Zheng, Q., Woehl, J. L., Solania, A., Chen, E., Dillon, N., Hull, M. V., Kotaniguchi, M., Cappiello, J. R., Kitamura, S., Nizet, V., Sharpless, K. B., & Wolan, D. W. (2020). Sulfur(VI) Fluoride Exchange (SuFEx)-Enabled High-Throughput Medicinal Chemistry. Journal of the American Chemical Society, 142(25), 10899–10904. https://doi.org/10.1021/jacs.9b13652
Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model
Science / Aug 21, 2020
Rogers, T. F., Zhao, F., Huang, D., Beutler, N., Burns, A., He, W., Limbo, O., Smith, C., Song, G., Woehl, J., Yang, L., Abbott, R. K., Callaghan, S., Garcia, E., Hurtado, J., Parren, M., Peng, L., Ramirez, S., Ricketts, J., … Burton, D. R. (2020). Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model. Science, 369(6506), 956–963. https://doi.org/10.1126/science.abc7520
Engineering SARS-CoV-2 neutralizing antibodies for increased potency and reduced viral escape pathways
iScience / Sep 01, 2022
Zhao, F., Keating, C., Ozorowski, G., Shaabani, N., Francino-Urdaniz, I. M., Barman, S., Limbo, O., Burns, A., Zhou, P., Ricciardi, M. J., Woehl, J., Tran, Q., Turner, H. L., Peng, L., Huang, D., Nemazee, D., Andrabi, R., Sok, D., Teijaro, J. R., … Jardine, J. G. (2022). Engineering SARS-CoV-2 neutralizing antibodies for increased potency and reduced viral escape pathways. IScience, 25(9), 104914. https://doi.org/10.1016/j.isci.2022.104914
Antigen pressure from two founder viruses induces multiple insertions at a single antibody position to generate broadly neutralizing HIV antibodies
PLOS Pathogens / Jun 29, 2023
Joyce, C., Murrell, S., Murrell, B., Omorodion, O., Ver, L. S., Carrico, N., Bastidas, R., Nedellec, R., Bick, M., Woehl, J., Zhao, F., Burns, A., Barman, S., Appel, M., Ramos, A., Wickramasinghe, L., Eren, K., Vollbrecht, T., … Smith, D. M. (2023). Antigen pressure from two founder viruses induces multiple insertions at a single antibody position to generate broadly neutralizing HIV antibodies. PLOS Pathogens, 19(6), e1011416. https://doi.org/10.1371/journal.ppat.1011416
Synthetic development of a broadly neutralizing antibody against snake venom long-chain α-neurotoxins
Science Translational Medicine / Feb 21, 2024
Khalek, I. S., Senji Laxme, R. R., Nguyen, Y. T. K., Khochare, S., Patel, R. N., Woehl, J., Smith, J. M., Saye-Francisco, K., Kim, Y., Misson Mindrebo, L., Tran, Q., Kędzior, M., Boré, E., Limbo, O., Verma, M., Stanfield, R. L., Menzies, S. K., Ainsworth, S., Harrison, R. A., … Jardine, J. G. (2024). Synthetic development of a broadly neutralizing antibody against snake venom long-chain α-neurotoxins. Science Translational Medicine, 16(735). https://doi.org/10.1126/scitranslmed.adk1867
Broadening a SARS-CoV-1–neutralizing antibody for potent SARS-CoV-2 neutralization through directed evolution
Science Signaling / Aug 15, 2023
Zhao, F., Yuan, M., Keating, C., Shaabani, N., Limbo, O., Joyce, C., Woehl, J., Barman, S., Burns, A., Tran, Q., Zhu, X., Ricciardi, M., Peng, L., Smith, J., Huang, D., Briney, B., Sok, D., Nemazee, D., Teijaro, J. R., … Jardine, J. G. (2023). Broadening a SARS-CoV-1–neutralizing antibody for potent SARS-CoV-2 neutralization through directed evolution. Science Signaling, 16(798). https://doi.org/10.1126/scisignal.abk3516
Fully synthetic platform to rapidly generate tetravalent bispecific nanobody–based immunoglobulins
Proceedings of the National Academy of Sciences / Jun 05, 2023
Misson Mindrebo, L., Liu, H., Ozorowski, G., Tran, Q., Woehl, J., Khalek, I., Smith, J. M., Barman, S., Zhao, F., Keating, C., Limbo, O., Verma, M., Liu, J., Stanfield, R. L., Zhu, X., Turner, H. L., Sok, D., Huang, P.-S., Burton, D. R., … Jardine, J. G. (2023). Fully synthetic platform to rapidly generate tetravalent bispecific nanobody–based immunoglobulins. Proceedings of the National Academy of Sciences, 120(24). https://doi.org/10.1073/pnas.2216612120
Education
University of Missouri
B.S., Medicinal Chemistry / May, 2011
University of Missouri–Kansas City
M.S., Cell and Molecular Biology / May, 2013
Kansas State University
Ph.D., Biochemistry and Molecular Biophysics / May, 2017
Experience
Scripps Research
Postdoctoral Fellow / June, 2017 — March, 2020
• Elucidate the host and/or microbiome targets of anti-proteolytic proteins produced in response to distal gut inflammation • Development of covalent neutrophil elastase inhibitor/biochemical probe through small molecule sulfonyl fluoride/fluorosulfate screen • Development and mechanistic understanding of small molecule inhibitor(s) of streptopain from S. pyogenes; followed by a high-throughput lead optimization strategy using SuFEx-based chemistry
International AIDS Vaccine Initiative
Analytical Scientist I/II / April, 2020 — October, 2023
• Characterization of protein/antibody preparations using biophysical methodology • Determination of kinetic properties of antibody/antigen interactions • Antigen screening through novel, synthetic yeast-display antibody and nanobody libraries • Affinity maturation of neutralizing antibodies through yeast-display methods • Development of fluorescent pseudovirus system for library-on-library screening
Scripps Research/Calibr
Sr. Staff Scientist / October, 2023 — Present
• Characterization of protein/antibody preparations using biophysical methodology • Determination of kinetic properties of antibody/antigen interactions • Antigen screening through novel, synthetic yeast-display antibody and nanobody libraries • Affinity maturation of neutralizing antibodies through yeast-display methods
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