Daniel Arroyo
Ph.D. candidate in human immunology and microbiology. 9 Years of molecular and cellular biology research experience. 1 year of clinical experience with knowledge of best practices.
About
Education
University of Utah
PhD Canidate, Immunology, microbiology, molecular biology, structural biology / May, 2029 (anticipated)
Experience
BioDot
Field Application Specialist / September, 2023 — Present
Performed feasibility studies with automated liquid handlers for picoliter, nanoliter and microliter dispensing • Assisted R&D to further characterize products and produce liquid class reference • Led lectures for various topics to educating teams from marketing, applications, and QC on NGS, Lyophilization and microscopy • Lyobead and lyophilization subject matter expert
Thermo Fisher Scientific
Scientist II / October, 2022 — June, 2023
• Research and development for CRISPR guided base editor and optimization, feasibility in various cell types, and assay development • Performed a wide variety of molecular techniques required by project: molecular cloning, mutagenesis, sequencing analysis, cell culturing, FPLC purification, western blot analysis, SDS-Page analysis, PCR, mRNA generation, transfections and base editing assays • Gene editing exposure to TALENs, CRISPER, Base Editors, Primer Editors
Curative
Associate Scientist / January, 2022 — September, 2022
• Assay development of a rapid (10 mins) multiplex RT-qPCR assay for Covid-19 diagnostics • Multidisciplinary project for EUA & 510K submission • Communicated with vendors and engineers over project improvements • Proposed and designed experiments • Performed single and multi-variable analysis • Rapid RT-qPCR Device SME (Subject Matter Expect) • Lab Safety Officer for department
Perkin Elmer
NGS Supervisor / November, 2020 — January, 2022
• Oversaw the high-throughput processing of up to 900 SARS-CoV-2 NGS samples per night • Managed a team of 22 individuals and facilitated their training from manual to fully automated operations • Trouble shot instruments and implemented procedures to improve production • Enforced SOP, SDS guidelines, GLP & GDP • Determined quality of DNA libraries produced by the team • Operated Illumina sequencers and facilitated individual’s training: MiSeq & NovaSeq 6000
California state University Fullerton, Biology Department
Research Associate / May, 2015 — December, 2019
• Analyzed high-throughput drug screening experiments, endogenous tagging through CRISPR/Cas9, and characterized knock-out cell lines by recording differences in cell invasion, differentiation, and intracellular replication • Performed protein expression and purification through affinity HPLC & gravity LC • Performed and analyzed high through-put drug screening data • Used SDS and Western Blot techniques to search for protein-protein interactions • Lead the start of simulating molecular docking of compound unavailable to the lab against protein of interest
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