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Marco Rito-Palomares
Research Expertise
Molecular Medicine
Applied Microbiology and Biotechnology
Analytical Chemistry
Filtration and Separation
Waste Management and Disposal
Renewable Energy, Sustainability and the Environment
Fuel Technology
Organic Chemistry
Inorganic Chemistry
Biotechnology
Pollution
Industrial and Manufacturing Engineering
Process Chemistry and Technology
Energy Engineering and Power Technology
Physical and Theoretical Chemistry
Clinical Biochemistry
Biochemistry
Cell Biology
Food Science
Bioengineering
Biomedical Engineering
Plant Science
Aquatic Science
Drug Discovery
Condensed Matter Physics
Fluid Flow and Transfer Processes
Colloid and Surface Chemistry
Molecular Biology
Structural Biology
Spectroscopy
Electrochemistry
Biochemistry (medical)
Agronomy and Crop Science
Nutrition and Dietetics
Environmental Engineering
Environmental Chemistry
Legacy Map
Full ViewPublications
Aqueous Two‐Phase Systems at Large Scale:
Challenges and Opportunities
Biotechnology Journal
2018
Aqueous two-phase systems for the recovery
and purification of phage therapy
products: Recovery of salmonella
bacteriophage ϕSan23 as a case study
Separation and Purification
Technology
2019
Cell‐based aqueous two‐phase systems for
therapeutics
Journal of Chemical Technology &
Biotechnology
2019
Continuous aqueous two-phase extraction of
microalgal C-phycocyanin using a coiled
flow inverter
Chemical Engineering and Processing -
Process Intensification
2019
Economic evaluation of the development of
a phage therapy product for the control of
Salmonella in poultry
Biotechnology Progress
2019
Ex vivo Manufactured Neutrophils for
Treatment of Neutropenia—A Process
Economic Evaluation
Frontiers in Medicine
2019
Strategies based on aqueous two-phase
systems for the separation of laccase from
protease produced by Pleurotus ostreatus
Fluid Phase Equilibria
2019
Thermo-separating polymer-based aqueous
two-phase systems for the recovery of
PEGylated lysozyme species
Journal of Chromatography B
2019
Advantages, Disadvantages and
Modifications of Conventional ELISA
SpringerBriefs in Applied Sciences and
Technology
2017
Economic evaluation of the primary
recovery of tetracycline with traditional
and novel aqueous two-phase systems
Separation and Purification
Technology
2018
Evaluation of the Detection Results
Obtained from ELISA
SpringerBriefs in Applied Sciences and
Technology
2017
Factorial and Economic Evaluation of an
Aqueous Two-Phase Partitioning Pilot Plant
for Invertase Recovery From Spent Brewery
Yeast
Frontiers in Chemistry
2018
Fundamentals and History of ELISA: The
Evolution of the Immunoassays Until
Invention of ELISA
SpringerBriefs in Applied Sciences and
Technology
2017
General Overviews on Applications of ELISA
SpringerBriefs in Applied Sciences and
Technology
2017
Improved recovery of bacteriophage M13
using an ATPS‐based bioprocess
Biotechnology Progress
2018
Is Bioseparation still the limiting step
in bioprocess development?
Journal of Chemical Technology &
Biotechnology
2018
Monte Carlo economic analysis of Baker's
yeast invertase purification using two‐
and three‐phase partitioning
Journal of Chemical Technology &
Biotechnology
2018
Novel aspects and future trends in the use
of aqueous two‐phase systems as a
bioengineering tool
Journal of Chemical Technology &
Biotechnology
2017
Practical experiences from the bench‐scale
implementation of a bioprocess for
fucoxanthin production
Journal of Chemical Technology &
Biotechnology
2017
Simulation of mono‐PEGylated lysozyme
separation in heparin affinity
chromatography using a general rate model
Journal of Chemical Technology &
Biotechnology
2017
Step by Step with ELISA: Mechanism of
Operation, Crucial Elements, Different
Protocols, and Insights on Immobilization
and Detection of Various Biomolecular
Entities
SpringerBriefs in Applied Sciences and
Technology
2017
Affinity ATPS Strategies for the Selective
Fractionation of Biomolecules
Food Engineering Series
2017
Characterization of Aqueous Two-Phase
Systems and Their Potential New
Applications
Food Engineering Series
2017
General Concepts and Definitions of
Aqueous Two-Phase Systems
Food Engineering Series
2017
In Focus: Biotechnology and chemical
technology for biorefineries and biofuel
production
Journal of Chemical Technology &
Biotechnology
2017
Integration of cell harvest with
affinity-enhanced purification of
monoclonal antibodies using aqueous
two-phase systems with a dual tag ligand
Separation and Purification
Technology
2017
Monolithic chromatography: insights and
practical perspectives
Journal of Chemical Technology &
Biotechnology
2016
Optimized purification of mono‐PEGylated
lysozyme by heparin affinity
chromatography using response surface
methodology
Journal of Chemical Technology &
Biotechnology
2017
Partial purification of a
polygalacturonase from a new
Aspergillus sojae mutant and its
application in grape mash maceration
International Journal of Food Science
& Technology
2017
Perspectives and Future Trends in Aqueous
Two-Phase System-Based Technology
Food Engineering Series
2017
Preface
Ethical Dilemmas in Emergency
Medicine
Process Economics: Evaluation of
the Potential of ATPS as
a Feasible Alternative to Traditional
Fractionation Techniques
Food Engineering Series
2017
Recovery and primary purification of
bacteriophage M13 using aqueous two‐phase
systems
Journal of Chemical Technology &
Biotechnology
2017
Recovery of PEGylated and native lysozyme
using an in situ aqueous two‐phase
system directly from the PEGylation
reaction
Journal of Chemical Technology &
Biotechnology
2017
Refolding of laccase from Trametes
versicolor using aqueous two phase
systems: Effect of different additives
Journal of Chromatography A
2017
A microdevice assisted approach for the
preparation, characterization and
selection of continuous aqueous two-phase
systems: from micro to bench-scale
Lab on a Chip
2016
An integrated practical implementation of
continuous aqueous two‐phase systems for
the recovery of human IgG: From the
microdevice to a multistage bench‐scale
mixer‐settler device
Biotechnology Journal
2016
Covalent immobilization of antibodies for
the preparation of immunoaffinity
chromatographic supports
Separation Science and Technology
2016
Dielectrophoretic behavior of PEGylated
RNase A inside a microchannel with
diamond‐shaped insulating posts
ELECTROPHORESIS
2015
Economic analysis of pilot‐scale
production of B‐phycoerythrin
Biotechnology Progress
2016
Economic analysis of uricase production
under uncertainty: Contrast of
chromatographic purification and aqueous
two-phase extraction (with and without PEG
recycle)
Biotechnology Progress
2015
Elimination of contaminants from cell
preparations using aqueous two-phase
partitioning
Separation and Purification
Technology
2016
Flotation Immunoassay: Masking the Signal
from Free Reporters in Sandwich
Immunoassays
Scientific Reports
2016
Growth kinetics and fucoxanthin production
of Phaeodactylum tricornutum and
Isochrysis galbana cultures at different
light and agitation conditions
Journal of Applied Phycology
2015
Intensified fractionation of brewery yeast
waste for the recovery of invertase using
aqueous two‐phase systems
Biotechnology and Applied
Biochemistry
2015
Modelling of electrokinetic phenomena for
capture of PEGylated ribonuclease A in a
microdevice with insulating structures
Biomicrofluidics
2016
PEGylated protein separation using
different hydrophobic interaction
supports: Conventional and monolithic
supports
Biotechnology Progress
2016
Pleurotus ostreatuslaccase recovery
from residual compost using aqueous
two-phase systems
Journal of Chemical Technology &
Biotechnology
2016
Synthesis of adsorbents with dendronic
structures for protein hydrophobic
interaction chromatography
Journal of Chromatography A
2016
A systems engineering perspective on
process integration in industrial
biotechnology
Journal of Chemical Technology &
Biotechnology
2014
Aldehyde PEGylation of laccase from
Trametes versicolor in route to
increase its stability: effect on
enzymatic activity
Journal of Molecular Recognition
2015
Application of affinity aqueous two‐phase
systems for the fractionation of CD133+
stem cells from human umbilical cord blood
Journal of Molecular Recognition
2015
Continuous enzyme aqueous two-phase
extraction using a novel tubular
mixer-settler in multi-step
counter-current arrangement
Separation and Purification
Technology
2015
Economic analysis of royalactin production
under uncertainty: Evaluating the effect
of parameter optimization
Biotechnology Progress
2015
Impact of aqueous two‐phase system design
parameters upon the
in situ refolding and recovery of
invertase
Journal of Chemical Technology &
Biotechnology
2015
Insights on the downstream purification of
fucoxanthin, a microalgal carotenoid, from
an aqueous two-phase system stream
exploiting ultrafiltration
Journal of Applied Phycology
2014
A novel strategy for the purification of a
recombinant protein using ceramic
fluorapatite-binding peptides as affinity
tags
Journal of Chromatography A
2014
Application of Aqueous Two-Phase Systems
for the Recovery of Bioactive
Low-Molecular Weight Compounds
Separation Science and Technology
2014
Aqueous Two-Phase System Strategies for
the Recovery of Proteins from Plants
Methods in Molecular Biology
2014
Aqueous two-phase systems strategies to
establish novel bioprocesses for stem
cells recovery
Critical Reviews in Biotechnology
2013
Continuous aqueous two-phase systems
devices for the recovery of biological
products
Food and Bioproducts Processing
2014
Microretroreflector-Sedimentation
Immunoassays for Pathogen Detection
Analytical Chemistry
2014
Partition behavior of CD133+
stem cells from human umbilical cord blood
in aqueous two‐phase systems: In route to
establish novel stem cell primary recovery
strategies
Biotechnology Progress
2014
Partition behavior of fucoxanthin in
ethanol‐potassium phosphate two‐phase
systems
Journal of Chemical Technology &
Biotechnology
2014
Potential application of aqueous two‐phase
systems and three‐phase partitioning for
the recovery of superoxide dismutase from
a clarified homogenate of
K luyveromyces marxianus
Biotechnology Progress
2014
Recovery of major royal jelly protein 1
expressed inPichia pastorisin
aqueous two-phase systems
Journal of Chemical Technology &
Biotechnology
2014
Separation of PEGylated variants of
ribonuclease A and apo-α-lactalbumin via
reversed phase chromatography
Journal of Chromatography A
2014
Spermine Sepharose as a clustered-charge
anion exchange adsorbent
Journal of Chromatography A
2014
Toward improving selectivity in affinity
chromatography with PEG ylated
affinity ligands: The performance of
PEG ylated protein A
Biotechnology Progress
2014
A novel process for the recovery of
superoxide dismutase from yeast exploiting
electroextraction coupled to direct
sorption
Journal of Chemical Technology &
Biotechnology
2013
Case Studies in the Application of Aqueous
Two-Phase Processes for the Recovery of
High Value Biological Products
ACS Symposium Series
2013
Journal of Chemical Technology &
Biotechnology
2013
Effects of chemical modifications in the
partition behavior of proteins in aqueous
two‐phase systems: A case study with
RNase A
Biotechnology Progress
2013
Low‐abundant protein extraction from
complex protein sample using a novel
continuous aqueous two‐phase systems
device
Journal of Separation Science
2012
Practical non‐chromatography strategies
for the potential separation of
PEGylated RNase
A conjugates
Journal of Chemical Technology &
Biotechnology
2012
Proteome wide evaluation of the separation
ability of hydrophobic interaction
chromatography by fluorescent dye binding
analysis
Journal of Molecular Recognition
2013
Scaling-up of a B-phycoerythrin production
and purification bioprocess involving
aqueous two-phase systems: Practical
experiences
Process Biochemistry
2013
Advances and trends in the design,
analysis, and characterization of
polymer–protein conjugates for
“PEGylaided” bioprocesses
Analytical and Bioanalytical
Chemistry
2012
Aqueous two-phase affinity partitioning
systems: Current applications and trends
Journal of Chromatography A
2012
Current strategies and challenges for the
purification of stem cells
Journal of Chemical Technology &
Biotechnology
2011
Hydrophobic interaction chromatography for
purification of monoPEGylated RNase A
Journal of Chromatography A
2012
PEGylation, detection and chromatographic
purification of site-specific PEGylated
CD133-Biotin antibody in route to stem
cell separation
Journal of Chromatography B
2012
Recovery of crocins from saffron stigmas
(Crocus sativus) in aqueous two-phase
systems
Journal of Chromatography A
2012
Application of an aqueous two-phase
systems strategy for the potential
recovery of a recombinant protein from
alfalfa (Medicago sativa)
Separation and Purification
Technology
2011
Colorimetric protein quantification in
aqueous two-phase systems
Process Biochemistry
2011
Current advances in the
non‐chromatographic fractionation and
characterization of PEGylated proteins
Journal of Chemical Technology &
Biotechnology
2010
Oxidation of Polycyclic Aromatic
Hydrocarbons using Partially Purified
Laccase from Residual Compost of
Agaricus bisporus
Chemical Engineering &
Technology
2011
Potential application of aqueous two‐phase
systems for the fractionation of RNase A
and α‐Lactalbumin from their PEGylated
conjugates
Journal of Chemical Technology &
Biotechnology
2010
Quantification of RNase A and Its
PEGylated Conjugates on Polymer-Salt Rich
Environments Using UV Spectrophotometry
Analytical Letters
2011
Study of biomolecules partition
coefficients on a novel continuous
separator using polymer-salt aqueous
two-phase systems
Separation and Purification
Technology
2011
Application of Aqueous Two‐Phase Systems
for the Potential Extractive Fermentation
of Cyanobacterial Products
Chemical Engineering &
Technology
2009
Aqueous two-phase systems strategies for
the recovery and characterization of
biological products from plants
Journal of the Science of Food and
Agriculture
2010
Coupled Application of Aqueous Two-Phase
Partitioning and 2D-Electrophoresis for
Characterization of Soybean Proteins
Separation Science and Technology
2010
Potential of Aqueous Two-Phase Systems
constructed on flexible devices: Human
serum albumin as proof of concept
Process Biochemistry
2010
Characterization of electrokinetic
mobility of microparticles in order to
improve dielectrophoretic concentration
Analytical and Bioanalytical
Chemistry
2009
Characterization of green‐tissue protein
extract from alfalfa (Medicago sativa) exploiting a 3‐D technique
Journal of Separation Science
2009
Recovery of laccase from the residual
compost of Agaricus bisporus in aqueous
two-phase systems
Process Biochemistry
2009
Separation of PEGylated from unmodified
ribonuclease A using sepharose media
Separation and Purification
Technology
2009
Bioseparation: The limiting step in
bioprocess development
Journal of Chemical Technology &
Biotechnology
2008
Extraction and Purification of Bioproducts
and Nanoparticles using Aqueous Two‐Phase
Systems Strategies
Chemical Engineering &
Technology
2008
Performance characterization of an
insulator‐based dielectrophoretic
microdevice
ELECTROPHORESIS
2008
Practical approach to protein recovery by
countercurrent distribution in aqueous
two‐phase systems
Journal of Chemical Technology &
Biotechnology
2007
Practical experiences from the development
of aqueous two‐phase processes for the
recovery of high value biological products
Journal of Chemical Technology &
Biotechnology
2007
Processing of soybean (Glycine max)
extracts in aqueous two‐phase systems as a
first step for the potential recovery of
recombinant proteins
Journal of Chemical Technology &
Biotechnology
2007
Protein manipulation with insulator-based
dielectrophoresis and direct current
electric fields
Journal of Chromatography A
2008
Dielectrophoresis for the manipulation of
nanobioparticles
ELECTROPHORESIS
2007
Insulator Based Dielectrophoresis: Effects
of Bulk Medium Properties
ASME 5th International Conference on
Nanochannels, Microchannels, and
Minichannels
2007
Direct comparison between ion-exchange
chromatography and aqueous two-phase
processes for the partial purification of
penicillin acylase produced by E. coli
Journal of Chromatography B
2006
Improved recovery of B‐phycoerythrin
produced by the red microalga
Porphyridium cruentum
Journal of Chemical Technology &
Biotechnology
2006
New aqueous two‐phase systems based on
poly(ethylene oxide sulfide) (PEOS) and
potassium phosphate for the potential
recovery of proteins
Journal of Chemical Technology &
Biotechnology
2006
Rotavirus-like particles primary recovery
from insect cells in aqueous two-phase
systems☆
Journal of Chromatography B
2006
Simplified two-stage method to
B-phycoerythrin recovery from Porphyridium
cruentum☆
Journal of Chromatography B
2006
A novel pectin‐degrading enzyme complex
from Aspergillus sojaeATCC 20235 mutants
Journal of the Science of Food and
Agriculture
2014
Potential Aqueous Two-Phase Processes for
the Primary Recovery of Colored Protein
from Microbial Origin
Engineering in Life Sciences
2005
Bioprocess intensification: a potential
aqueous two-phase process for the primary
recovery of B-phycoerythrin from
Porphyridium cruentum
Journal of Chromatography B
2004
Practical application of aqueous two-phase
partition to process development for the
recovery of biological products
Journal of Chromatography B
2004
Recovery in aqueous two-phase systems of
lutein produced by the green microalga
Chlorella protothecoides
Journal of Chromatography B
2004
Aqueous two‐phase systems for the recovery
of a recombinant viral coat protein from
Escherichia coli
Journal of Chemical Technology &
Biotechnology
2002
Bioprocess Intensification: A Radical New
Process for Recovering Inclusion Body
Protein
Food and Bioproducts Processing
2002
Process integration using aqueous
two-phase partition for the recovery of
intracellular proteins
Chemical Engineering Journal
2002
Production and biotransformation of
6-pentyl-α-pyrone by Trichoderma harzianum
in two-phase culture systems
Applied Microbiology and
Biotechnology
2002
Practical application of aqueous two‐phase
systems for the development of a prototype
process for c‐phycocyanin recovery from
Spirulina maxima
Journal of Chemical Technology &
Biotechnology
2001
The potential application of aqueous
two-phase systems for in situ recovery of
6-pentyl-∞-pyrone produced by Trichoderma
harzianum
Enzyme and Microbial Technology
2001
Aroma compounds recovery from mycelial
cultures in aqueous two-phase processes
Journal of Chromatography B: Biomedical
Sciences and Applications
2000
Effect of biological suspensions on the
position of the binodal curve in aqueous
two-phase systems
Journal of Chromatography B: Biomedical
Sciences and Applications
2000
Generic application of an aqueous
two-phase process for protein recovery
from animal blood
Process Biochemistry
2000
Kinetics of phase separation under
different process and design parameters in
aqueous two-phase systems
Journal of Chromatography B: Biomedical
Sciences and Applications
2000
Practical implementation of aqueous
two-phase processes for protein recovery
from yeast
Journal of Chemical Technology &
Biotechnology
2000
Studies on the physical and compositional
changes in collapsing beer foam
Chemical Engineering Journal
1999
Influence of system and process parameters
on partitioning of cheese whey proteins in
aqueous two-phase systems
Journal of Chromatography B: Biomedical
Sciences and Applications
1998
Impact of cell disruption and polymer
recycling upon aqueous two-phase processes
for protein recovery
Journal of Chromatography B: Biomedical
Sciences and Applications
1996
Education
University of Birmingham
chemical engineering / June, 1995
Birmingham
Experience
Instituto Tecnológico y de Estudios Superiores de Monterrey
Dean TecSalud
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