Joseph Bucukovski, Ph.D.
Accomplished BIomedical Scientist with experience in biosensor development and microphysiological systems
Research Expertise
About
Publications
Array-based analysis of SARS-CoV-2, other coronaviruses, and influenza antibodies in convalescent COVID-19 patients
Biosensors and Bioelectronics / Dec 01, 2020
Steiner, D. J., Cognetti, J. S., Luta, E. P., Klose, A. M., Bucukovski, J., Bryan, M. R., Schmuke, J. J., Nguyen-Contant, P., Sangster, M. Y., Topham, D. J., & Miller, B. L. (2020). Array-based analysis of SARS-CoV-2, other coronaviruses, and influenza antibodies in convalescent COVID-19 patients. Biosensors and Bioelectronics, 169, 112643. https://doi.org/10.1016/j.bios.2020.112643
Biosensing with Silicon Nitride Microring Resonators Integrated with an On-Chip Filter Bank Spectrometer
ACS Sensors / Feb 14, 2023
Bryan, M. R., Butt, J. N., Bucukovski, J., & Miller, B. L. (2023). Biosensing with Silicon Nitride Microring Resonators Integrated with an On-Chip Filter Bank Spectrometer. ACS Sensors, 8(2), 739–747. https://doi.org/10.1021/acssensors.2c02276
A Multiplex Label-Free Approach to Avian Influenza Surveillance and Serology
PLOS ONE / Aug 04, 2015
Bucukovski, J., Latorre-Margalef, N., Stallknecht, D. E., & Miller, B. L. (2015). A Multiplex Label-Free Approach to Avian Influenza Surveillance and Serology. PLOS ONE, 10(8), e0134484. https://doi.org/10.1371/journal.pone.0134484
Crowd on a Chip: Label-Free Human Monoclonal Antibody Arrays for Serotyping Influenza
Analytical Chemistry / Jul 09, 2018
Zhang, H., Henry, C., Anderson, C. S., Nogales, A., DeDiego, M. L., Bucukovski, J., Martinez-Sobrido, L., Wilson, P. C., Topham, D. J., & Miller, B. L. (2018). Crowd on a Chip: Label-Free Human Monoclonal Antibody Arrays for Serotyping Influenza. Analytical Chemistry, 90(15), 9583–9590. https://doi.org/10.1021/acs.analchem.8b02479
Label-free microarray-based detection of autoantibodies in human serum
Journal of Immunological Methods / Aug 01, 2018
Bucukovski, J., Carter, J. A., Striemer, C. C., Müllner, S., Schulte-Pelkum, J., Schulz-Knappe, P., & Miller, B. L. (2018). Label-free microarray-based detection of autoantibodies in human serum. Journal of Immunological Methods, 459, 44–49. https://doi.org/10.1016/j.jim.2018.05.011
Optical sensors for coronavirus antibodies: Analyzing the human response to COVID-19
Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XXII / Apr 12, 2021
Miller, B. L., Cognetti, J., Steiner, D., Bryan, M., Klose, A., Luta, E., Bucukovski, J., Shanahan, C., Abedin, M., Tokranova, N., Young, E., Meinhart, C., Schmuke, J., Warren, H., Jakubowicz, R., & Cady, N. (2021). Optical sensors for coronavirus antibodies: Analyzing the human response to COVID-19. In J. A. Guicheteau & C. R. Howle (Eds.), Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XXII (p. 14). SPIE. https://doi.org/10.1117/12.2586109
Leveraging integrated photonic sensors to detect senescence-associated secreted proteins in a human tendon-on-chip model
Frontiers in Biological Detection: From Nanosensors to Systems XVI / Mar 13, 2024
Bucukovski, J. (2024). Leveraging integrated photonic sensors to detect senescence-associated secreted proteins in a human tendon-on-chip model. In B. L. Miller, S. M. Weiss, & A. Danielli (Eds.), Frontiers in Biological Detection: From Nanosensors to Systems XVI (p. 5). SPIE. https://doi.org/10.1117/12.2692672
Education
University of Rochester
Ph.D., Biophysics, Structural, and Computational Biology / August, 2024
University of Rochester
M.S., Biomedical Engineering / October, 2014
University of Rochester
B.S., Biomedical Engineering / May, 2012
Experience
Adarza BioSystems
Research Associate / June, 2015 — September, 2016
Senior Research Associate / September, 2016 — May, 2017
• Developed an autoantigen microarray for assaying Sjogren’s syndrome patient samples (published) • Conducted thorough assay development of antibody microarrays to detect systemic levels of chemokines and cytokines in human serum (> 30-plex) • Wrote and revised SOPs for analytical laboratory equipment operation and maintenance • Transferred assay processes and technical expertise to St. Louis manufacturing site • Performed stability testing of coated microarrays for long-term storage capabilities • Trained and mentored laboratory personnel in cleanroom and BSL-2 environments • User of all microarray fabrication printers, with proven ability to streamline, optimize, and troubleshoot scale-up processes • Developed novel data analysis methods to reduce analysis time, improve normalization techniques, and add automation to assay software package • Attended conference to present marketing poster to KOLs, potential customers, and investors based on product development work
Vaccinex, Inc.
Senior Research Associate / September, 2017 — August, 2019
• Lead Critical Reagent Program to ensure proper storage, stability, and inventory of GLP/GMP assay materials • Executed stability and release testing of Phase II drug candidate using bioanalytical methods and prepared technical QC/QA reports for management • Served as the subject matter expert for offsite CRO audit to ensure accuracy of bioanalytical test methods for clinical trials and relevant protocols / documentation • Optimized ELISA PK and ADA (immunogenicity) assays to be used by CRO for testing clinical samples, i.e. CSF, serum, buffer • Assisted with internal production duties such as DNA isolation from E. coli and large-scale transient transfection of CHO/HEK293 cell lines • Optimized ELISA assays to be used by CRO for testing clinical samples, i.e. CSF, serum, buffer • Researched putative biomarkers of neurodegenerative disease in literature and screened patient samples for prevalence of various cytokines, chemokines, and other proteins using Luminex
University of Rochester Medical Center
Graduate Research Assistant (Doctoral Candidate) / January, 2020 — August, 2024
• Designed microfluidic tissue-chip devices to incorporate human tenocyte-laden hydrogels and interrogate wound healing biology with integrated PhRR sensors for real-time cytokine and chemokine measurements (manuscript in progress) • Followed FDA guidelines in Bioanalytical Method Validation to systematically assess the quality and reliability of photonic sensor-derived bioassay data • Developing pneumococcal capsular polysaccharide photonic biosensors to characterize antibody response against serotypes of invasive disease
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