Joseph Bucukovski, Ph.D.
Rochester, New York, United States of
America
Accomplished Biomedical Scientist with
expertise in Organ-on-Chip (OoC) platforms
and immunoassay development
Experience
Adarza BioSystems
Research Associate / June, 2015 —
September, 2016
Senior Research
Associate / September, 2016 —
May, 2017
• Developed an autoantigen
microarray for assaying
Sjogren’s syndrome patient
samples (published) •
Conducted thorough assay
development of antibody
microarrays to detect systemic
levels of chemokines and
cytokines in human serum (>
30-plex) • Wrote and revised
SOPs for analytical laboratory
equipment operation and
maintenance • Transferred
assay processes and technical
expertise to St. Louis
manufacturing site • Performed
stability testing of coated
microarrays for long-term
storage capabilities • Trained
and mentored laboratory
personnel in cleanroom and
BSL-2 environments • User of
all microarray fabrication
printers, with proven ability
to streamline, optimize, and
troubleshoot scale-up
processes • Developed novel
data analysis methods to
reduce analysis time, improve
normalization techniques, and
add automation to assay
software package • Attended
conference to present
marketing poster to KOLs,
potential customers, and
investors based on product
development work
Vaccinex, Inc.
Senior Research
Associate / September, 2017 —
August, 2019
• Lead Critical Reagent
Program to ensure proper
storage, stability, and
inventory of GLP/GMP assay
materials • Executed stability
and release testing of Phase
II drug candidate using
bioanalytical methods and
prepared technical QC/QA
reports for management •
Served as the subject matter
expert for offsite CRO audit
to ensure accuracy of
bioanalytical test methods for
clinical trials and relevant
protocols / documentation •
Optimized ELISA PK and ADA
(immunogenicity) assays to be
used by CRO for testing
clinical samples, i.e. CSF,
serum, buffer • Assisted with
internal production duties
such as DNA isolation from E.
coli and large-scale transient
transfection of CHO/HEK293
cell lines • Optimized ELISA
assays to be used by CRO for
testing clinical samples, i.e.
CSF, serum, buffer •
Researched putative biomarkers
of neurodegenerative disease
in literature and screened
patient samples for prevalence
of various cytokines,
chemokines, and other proteins
using Luminex
University of Rochester Medical
Center
Graduate Research Assistant
(Doctoral Candidate) / January, 2020 —
August, 2024
• Designed microfluidic
tissue-chip devices to
incorporate human
tenocyte-laden hydrogels and
interrogate wound healing
biology with integrated PhRR
sensors for real-time cytokine
and chemokine measurements
(manuscript in progress) •
Followed FDA guidelines in
Bioanalytical Method
Validation to systematically
assess the quality and
reliability of photonic
sensor-derived bioassay data •
Developing pneumococcal
capsular polysaccharide
photonic biosensors to
characterize antibody response
against serotypes of invasive
disease
ACM Global Laboratories
Associate Principal
Scientist / September, 2024 —
Present
• Led and performed global
laboratory validation of
enzymatic creatinine assay for
the analysis of human clinical
trials subjects • Developed a
working list of >100
clinical laboratory test
calculations for regulatory
review and approval •
Consulting for two ongoing
clinical trial studies
requiring pathology and
LC-MS/MS mediated screening
assays for patient enrollment
• Provide support to
Scientific Affairs Pre-Award
team in clinical
protocol/synopsis
interpretation • Responsible
for scientific oversight of
partner lab specializing in
biomarker clinical trial
assays with an emphasis on
PD/PK and esoteric testing •
Increased capacity of ACM test
catalog to include 5
additional test calculations
and reference ranges after
client feedback • Conducted a
remote audit of partner
laboratory to assess the
technical operations after
site relocation •
Collaborating with partner lab
to optimize shipping and
storage conditions of duodenal
biopsies
Research Expertise
Optical Biosensors
Immunology
Bio-photonics
Organ-on-Chip Models
Microphysiological Systems
And 1 more
About
Biomedical Research Scientist with 10+
years of proven results. I'm
passionate about using technology to
improve human health. I thrive in
taking on challenging projects both in
and out the lab and excel as a
scientific communicator. My area(s) of
expertise is the development of novel
immunoassay methods for clinically
relevant targets and Organ-on-Chip
models to study disease processes and
therapeutic efficacy.